Simplified cell labelling with indium-111 acetylacetonate and indium-111 oxinate

Abstract

Cell labelling by means of chelated 111In has been reported to be a useful clinical tool in the diagnosis of abscesses (Thakur et a1., 1977). 111In oxinate (oxine) can label the cellular blood components granulocytes (Segal et al., 1976; Goedemans et al., 1980), lymphocytes (Lavender et al., 1977), platelets (Thakur et al., 1976; McIlmoyle et al., 1977; Wistow et al., 1978; Scheffel et al., 1979) and erythrocytes (Thakur, 1977). These cells have been used to locate abscesses and inflammatory reactions, myocardial infarctions, thrombus formation, rejection of transplanted organs etc. For some time, labelled erythrocytes have been extensively used for blood volume measurement. 111In-labelled erythrocytes show cardiac, liver and spleen blood pool activity 24 hours after intravenous administration. 111In oxinate red cells provide a potential method for detection of intermittent gastrointestinal bleeding (Winzelberg et al., 1980). It has been suggested (Sinn and Silvester, 1979) that acetylacetone has an advantage over oxine with regard to cell toxicity due to the presence of ethanol in the oxine preparation and also that oxine itself could be toxic at high concentrations.