Monitoring of acute axonal injury in the swine spinal cord with EAE by diffusion tensor imaging

Abstract

PURPOSE: To evaluate the ability of diffusion tensor imaging (DTI) to detect and monitor acute axonal injury in swine spinal cord with acute experimental allergic encephalomyelitis (EAE). MATERIALS AND METHODS: Magnetic resonance imaging of the cervical spinal cord was performed in vivo at different time points through the onset and progression of EAE using a 3 Tesla clinical scanner. The DTI parameters were calculated in four separate regions of interest at the C2/C3 level. The quantitative DTI-pathology and DTI-clinical correlations were verified. RESULTS: In the monophasic acute course of EAE onset and progression, axial diffusivity (AD) decrease correlates with acute axonal injury (r = -0.84; P < 0.001). By contrast, radial diffusivity does not change and no demyelination in histopathology was detected. Moreover, a clear correlation between clinical disease and axial diffusivity was found in two swine EAE models (r = -0.86; P < 0.001 and r = -0.92; P < 0.001). CONCLUSION: AD corresponds with axonal injury in the absence of demyelination and may be a useful noninvasive tool to investigate the underlying pathogenic processes of multiple sclerosis and to monitor the effects of experimental treatments for axonal injury.