Identifying Natural Product Biosynthetic Genes from a Soil Metagenome by Using T7 Phage Selection

Abstract

Doing the dirty work: Metagenomic DNA was directly isolated from soil bacteria to construct a T7 phage library. Iterative phage selection by protein modification catalyzed by phosphopantetheinyl transferase enriched putative acyl carrier protein and peptidyl carrier protein genes that are associated with natural product biosynthetic enzymes. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.