Biotechnology and Applied Biochemistry · 1999 · 156 citations · 11 references
Life SciencesBiotechnologyPlant BiologyMolecular BiotechnologyPlant BiotechnologyTransgenic CropsAgrobacterium-mediated TransformationPlant Gene ExpressionPlant VirusesMolecular FarmingPlant-based Protein ExpressionTransient ExpressionTransient Expression SystemsTransient Expression VectorsTransient Gene ExpressionViral Expression VectorsViral Vectors
Molecular farming in plants can be achieved by stable or transient expression of a recombinant protein. Transient expression of recombinant proteins in plants can rapidly provide large amounts of the proteins for detailed characterization. It is fast, flexible and can be carried out at field scale using viral vectors, but it lacks the increases in production volume that can be achieved easily with stable transgenic crops. This review article focuses on discussing the applications of transient expression using viral vectors, biolistic methods or agroinfiltration.
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An Agrobacterium-mediated transient gene expression system for intact leaves
Jyoti Kapila, Riet De Rycke, Marc Van Montagu et al. · Plant Science · 1997 · 727 citations
High Efficiency Transformation of Cultured Tobacco Cells
Gynheung An · PLANT PHYSIOLOGY · 1985 · 458 citations · Full text