Towards lysozyme nanotube and 3D hybrid self-assembly

Abstract

We report lysozyme self-assembly into nanotubes, under the effect of hydrolysis at pH 2 and 90 °C. We resolve the final steps of the fibrillation pathway, entailing the closure of multi-stranded helical ribbons into nanotubes, and we provide evidence of β-sheet arrangement within the nanotubes, demonstrating amyloid-like aggregation. Addition of chloroauric acid to the self-assembled structures can lead to generation of either gold single crystal nanoplatelets or gold nanoparticles (when a reducing agent is added) decorating the nanotube and ribbon surfaces. The crystal-based organic-inorganic hybrids further assemble into 3D "sandwiched" structures.