Oxidative capacity and ageing in human muscle

TLDR

The study quantified how oxidative capacity per muscle volume declines with age in human vastus lateralis. Oxidative capacity was estimated from 31P MR spectroscopy of phosphocreatine recovery and muscle biopsies to assess mitochondrial volume density, using a simple electrical circuit model of respiratory control. Elderly subjects had about a 50 % lower oxidative capacity per muscle volume, driven by reduced mitochondrial content and lower mitochondrial oxidative capacity.

Abstract

1 This study determined the decline in oxidative capacity per volume of human vastus lateralis muscle between nine adult (mean age 38.8 years) and 40 elderly (mean age 68.8 years) human subjects (age range 25-80 years). We based our oxidative capacity estimates on the kinetics of changes in creatine phosphate content ([PCr]) during recovery from exercise as measured by 31P magnetic resonance (MR) spectroscopy. A matched muscle biopsy sample permitted determination of mitochondrial volume density and the contribution of the loss of mitochondrial content to the decline in oxidative capacity with age. 2 The maximal oxidative phosphorylation rate or oxidative capacity was estimated from the PCr recovery rate constant (kPCr) and the [PCr] in accordance with a simple electrical circuit model of mitochondrial respiratory control. Oxidative capacity was 50 % lower in the elderly vs. the adult group (0.61 ± 0.04 vs. 1.16 ± 0.147 mM ATP s−1). 3 Mitochondrial volume density was significantly lower in elderly compared with adult muscle (2.9 ± 0.15 vs. 3.6 ± 0.11 %). In addition, the oxidative capacity per mitochondrial volume (0.22 ± 0.042 vs. 0.32 ± 0.015 mM ATP (s %)−1) was reduced in elderly vs. adult subjects. 4 This study showed that elderly subjects had nearly 50 % lower oxidative capacity per volume of muscle than adult subjects. The cellular basis of this drop was a reduction in mitochondrial content, as well as a lower oxidative capacity of the mitochondria with age.