Chromatographic Separation of Puberty Accelerating Pheromone from Male Mouse Urine1

Abstract

Dialysis and sequential ultrafiltration were used to separate urine from adult male mice into a number of fractions, all of which proved to be capable of accelerating the sexual maturation of female mice. Chromatography of the lowest molecular weight fraction on Sephadex G-15 yielded several fractions, only one of which was active. The active fraction was eluted at a position corresponding to a molecular weight of 860. It contained no detectable steroids but gave a strong positive test for peptides.