2′‐<i>N</i>‐(Pyren‐1‐yl)acetyl‐2′‐Amino‐α‐L‐LNA: Synthesis and Detection of Single Nucleotide Mismatches in DNA and RNA Targets

Abstract

Precise positioning of intercalators furnishes a SNP-detection tool. The conformationally locked 2-oxo-5-azabicyclo-[2.2.1]heptane skeleton of 2′-amino-α-L-LNA monomer X directs the N2′-linked pyrene moiety into nucleic acid duplex cores to give highly stabilized duplexes. We have used this precise positioning of pyrene moieties to develop probes that signal the presence of single-nucleotide mismatches in DNA/RNA targets by excimer signal formation. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2007/z700144_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.