Achieving Secondary Structural Resolution in Kinetic Measurements of Protein Folding: A Case Study of the Folding Mechanism of Trp‐cage

Abstract

A new twist: A multi-probe and multi-frequency approach is shown for dissecting the folding dynamics of individual protein structural elements. In response to a temperature jump the 310-helix (blue in the picture) of the miniprotein Trp-cage unfolds before the global unfolding of the protein, whereas the formation of the cage structure depends on the folding of the α-helix (red).