Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Common Strains of<i>Escherichia coli</i>

Abstract

We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.