Real‐Time Monitoring of DNA Polymerase Function and Stepwise Single‐Nucleotide DNA Strand Translocation through a Protein Nanopore

Abstract

Stepping it forward: A major challenge in realizing single-molecule nanopore DNA sequencing is to devise a way to ratchet DNA strands through a nanopore at appropriate speed that is commensurate with real-time sequential nucleobase identification. A promising approach is presented that exploits the intrinsic motor function of DNA polymerases to effect DNA strand translocation through an α-hemolysin nanopore (see picture). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.