Obihiro University of Agriculture and Veterinary Medicine Institutional Repository · 2006 · 13 citations · 19 references
Open access
Veterinary MedicineInfectious DiseasesPathobiologyMolecular DiagnosticsProtozoologyVeterinary Parasitic DiseasesAntigen Detection AssaysMolecular Diagnosis Of ParasitesParasitic Disease DiagnosisSerological DiagnosisSerological DiagnosticsVeterinary ImmunologyAntigen Detection TestsDiagnostic AntigensImmunodiagnostic AssaysImmunodominant AntigensSerological AntigensSerological Detection Of NematodesValidation Of Diagnostic TestsWestern Blot Analysis
Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The\ndense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the\npresent study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was\nexpressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay\n(ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and\n-negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined\nfor the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95\n(9.47%) samples from Japan and China, respectively, were positive for antibodies to N. caninum. Of the 32\nELISA-positive samples, 29 (90.62%) were confirmed as positive by Western blot analysis with whole\ntachyzoite antigens. These results indicate that the recombinant NcGRA7t would be a reliable antigen in\nELISA for the diagnosis of canine neosporosis.
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Redescription of Neospora caninum and its differentiation from related coccidia
J. P. Dubey, B. C. Barr, John R. Barta et al. · International Journal for Parasitology · 2002 · 271 citations
Life Sciences, Biology, Botany +10