EVALUATION OF IN VITROANTICANCER ACTIVITY AND GC-MS ANALYSIS FROM LEAF SOPHORA INTERRUPTA BEDD

Abstract

Objective: Sophora interrupta Bedd (Fabaceae) is one of the well-known medicinal herb used in folk medicine for the treatment of cancer and inflammatory associated diseases. In this paper we aimed to evaluate the antioxidant and anti-cancer, properties of aqueous, methanol and n-hexane leaf extracts. Methods: The leaf extracts were analyzed for antioxidant activity using DPPH free radical scavenging assay and anticancer activity by measuring the cell viability using MCF-7 and PC-3 cancer cell lines. GC-MS analysis was performed to identify anti-cancer compounds present in the active leaf extract. Results: The polar solvent extracts showed good antioxidant at 500 μg/ml as related to non-polar solvents. Moreover, methanol extract exhibited highest percentage of cell death, in both MCF-7 (IC 50 500 μg/ml) and PC-3 cells (IC 50 1000 μg/ml), which is also evident from morphological observations, acridine orange and ethidium bromide dye exclusion assays. Conclusion: Over all, it suggests that leaf methanol extract contain anticancer compounds, which are evident from our GC-MS analysis.