Lanthanide‐Coded Protease‐Specific Peptide–Nanoparticle Probes for a Label‐Free Multiplex Protease Assay Using Element Mass Spectrometry: A Proof‐of‐Concept Study

Abstract

Coded messages: Lanthanide (Ln) coded protease-specific peptide–nanoparticle (NPs) conjugates act as probes for a multiplex protease assay (MPA) using inductively coupled plasma mass spectrometry (ICP-MS; see picture). MPA can be achieved by centrifugal separation of the protease-cleaved and uncleaved probes and subsequent determination of the released Ln tags. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.