Chill-Induced Decrease in Capacity of RuBP Carboxylation and Associated H2O2 Accumulation in Cucumber Leaves are Alleviated by Grafting onto Figleaf Gourd

Abstract

Grafted plants showed a significantly higher light-saturated rate of CO(2) assimilation (A(sat)) than own-rooted plants when roots were gradually cooled, but no differences were detected when shoots were cooled. Chill at 7 degrees C irreversibly reduced A(sat), and significantly decreased maximum carboxylation activity, Rubisco content and initial Rubisco activity. However, grafted plants showed weaker inhibition, together with decreased electron flux in the water-water cycle. Higher activity of antioxidant enzymes with less ROS production was found in grafted plants. In addition, ABA concentration increased by 48.4-fold whilst cytokinin concentration decreased by 91.5% in the xylem sap of own-rooted plants after exposure to a 7 degrees C chill. In comparison, ABA and cytokinin concentrations increased by 10.5-fold and 36.9%, respectively, for the grafted plants. Improved plant growth was also observed in grafted plants after the chill. These results suggest that some signals coming from chilling-resistant roots (i.e. ABA and cytokinins) protect leaf photosynthesis in shoots of chilling-sensitive plants.