Direct Polymerase Synthesis of Reactive Aldehyde‐Functionalized DNA and Its Conjugation and Staining with Hydrazines

Abstract

Reactive aldehyde-modified DNA was prepared in two steps by Suzuki cross-coupling of halogenated nucleoside triphosphates (dNTPs) with 4-formylthiophene-2-boronic acid and subsequent polymerase incorporation of the modified nucleotides into DNA (see scheme; PEX=primer extension, PCR=polymerase chain reaction). Formation of hydrazones with arylhydrazines under aqueous conditions was used for DNA staining.