Biosynthesis of the Cyclotide Kalata B1 by Using Protein Splicing

Abstract

The tides are turning: Kalata B1 (KB1) is the prototypical member of an exceptionally stable and resistant family of plant-derived miniproteins called cyclotides. The biosynthesis of KB1 and a small library of KB1-based cyclotides (variation occurs in five solvent-exposed loops) in E. coli has been performed by using recombinant-DNA expression techniques and protein splicing.