Concepedia

Calcium-Dependent and -Independent Phosphoenolpyruvate Carboxylase Kinases in Sorghum Leaves: Further Evidence for the Involvement of the Calcium-Independent Protein Kinase in the in situ Regulatory Phosphorylation of C4 Phosphoenolpyruvate Carboxylase

Mohamed Nhiri, Naı̈ma Bakrim, V. Pacquit, Zakia El Hachimi-Messouak, L. Osuna, J. Vidal

Plant and Cell Physiology · 1998 · 22 citations · 0 references

Concepts

Life SciencesPlant BiologyPlant SciencesPlant BiochemistryPlant Molecular BiologyPlant PhysiologyC4 PhotosynthesisChloroplast BiochemistryPlant CellsPlant EnzymesPlant MetabolismProtoplast CultureIsolation Of Mesophyll ProtoplastsMesophyll Cell FunctionPhosphoenolpyruvate Carboxykinase RegulationPhosphoenolpyruvate Carboxylase RegulationPhosphorylation CascadesPlant Carbohydrate Metabolism

Abstract

Calcium-dependent phosphoenolpyruvate carboxylase protein kinase was copurified with C4 phosphoenolpyruvate carboxylase (C4 PEPC) from illuminated Sorghum leaves during purification by various procedures. Isolated mesophyll cell protoplasts contained both calcium-dependent and -independent protein kinases. The latter was induced by light and weak bases and was found to be the major protein kinase phosphorylating C4 PEPC in the mesophyll.