BioTechniques · 2000 · 32 citations · 21 references
MedicineMolecular BiologyPathologyDiagnostic PathologyNucleic Acid ExtractionPolymerase Chain ReactionSpecimen PreparationSpecimen ProcessingDna ExtractionExtraction From Formalin-fixed Paraffin-embedded SamplesNucleic Acid Sample PreparationPcr-based DetectionPolymerase Chain Reaction (Pcr) AssaysTissue Nucleic Acid ExtractionTotal Nucleic Acid Extraction
achieved by a few days incubation at room temperature (5) or 2-4 h incubation at 42 C in the cell/PEG/Li-mixture.
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Transformation of intact yeast cells treated with alkali cations
Hisao Ito, Y Fukuda, Kousaku Murata et al. · Journal of Bacteriology · 1983 · 7.7K citations · Full text
Studies on the transformation of intact yeast cells by the LiAc/SS‐DNA/PEG procedure
R. Daniel Gietz, Robert H. Schiestl, Andrew Willems et al. · Yeast · 1995 · 2.1K citations