Molecular Characterization of Genetic Loci Required for Secretion of Exoproducts in <i>Burkholderia pseudomallei</i>

Abstract

Previous studies have demonstrated that Burkholderia pseudomallei secretes protease, lipase, and phospholipase C (PLC) into the extracellular milieu, but their mechanisms of secretion and roles in pathogenesis have not been elucidated. In this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and PLC.