Rapid analysis of plant gene expression by a novel reverse transcriptase‐PCR method

Abstract

A recently described reverse transcriptase‐polymerase chain reaction (RT‐PCR) where one oligonucleotide primer is end‐labelled has been applied to the analysis of expression of UsnRNA genes and a U2B″ protein coding gene from potato. This rapid, sensitive and accurate method of expression analysis can be applied to the study of the expression of any plant gene for which the sequence is known, in either wild‐type plants, transgenics or transfected protoplasts.