International Journal of Environmental Health Research · 2013 · 17 citations · 28 references
PharmacyToxicologyEnvironmental ToxicologyIndustrial ToxicologyOccupational ToxicologyComet AssayDna DamageDna Strand BreaksGenotoxicity AssessmentGenotoxicity TestingOccupational Genotoxic ExposureAlkaline Comet AssayAsphalt MixturesDna Damage InductionGel ElectrophoresisGenotoxic Exposure AssessmentLymphocyte Comet AssayLymphocyte Genotoxic DamagePolycyclic Aromatic Hydrocarbons
Asphalt has a highly complex structure and it contains several organic compounds including polycyclic aromatic hydrocarbons and heterocyclic compounds. In this study, comet assay was used to detect the DNA damage in blood lymphocytes of 30 workers exposed to asphalt fumes and 30 nonexposed controls. This is the first report on Turkish asphalt workers' investigated DNA damage using the alkaline single cell gel electrophoresis (SCGE). The DNA damage was evaluated by the percentage of DNA in the comet tail (% tail DNA) for each cell. According to our results, workers exposed to asphalt fumes had higher DNA damage than the control group (p < 0.01). The present study showed that asphalt fumes caused a significant increase in DNA damage and the comet assay is a suitable method for determining DNA damage in asphalt workers.
28
Recommendations for conducting the in vivo alkaline Comet assay
Andreas Hartmann · Mutagenesis · 2002 · 1.1K citations · Full text