Tonic inhibition in mouse hippocampal CA1 pyramidal neurons is mediated by α5 subunit-containing γ-aminobutyric acid type A receptors

TLDR

GABA is the principal inhibitory neurotransmitter in the brain, regulating memory through transient IPSCs, and GABA A receptors that mediate tonic conductance are highly sensitive to low ambient GABA levels. The study aims to describe a nonsynaptic, tonic form of inhibition in mouse CA1 pyramidal neurons generated by a distinct subpopulation of GABA A receptors. This tonic inhibitory conductance is predominantly mediated by α5 subunit‑containing GABA A receptors, which possess unique pharmacological and kinetic properties compared to postsynaptic receptors. The tonic current is highly sensitive to amnestic drug enhancement, and because α5GABA A receptors are restricted to the hippocampus and their reduced function is linked to improved memory, the data suggest that α5GABA A‑mediated tonic inhibition in CA1 pyramidal neurons is essential for cognitive processes.

Abstract

The principal inhibitory neurotransmitter in the mammalian brain, γ-aminobutyric acid (GABA), is thought to regulate memory processes by activating transient inhibitory postsynaptic currents. Here we describe a nonsynaptic, tonic form of inhibition in mouse CA1 pyramidal neurons that is generated by a distinct subpopulation of GABA type A receptors (GABA A Rs). This tonic inhibitory conductance is predominantly mediated by α5 subunit-containing GABA A Rs (α5GABA A Rs) that have different pharmacological and kinetic properties compared to postsynaptic receptors. GABA A Rs that mediate the tonic conductance are well suited to detect low, persistent, ambient concentrations of GABA in the extracellular space because they are highly sensitive to GABA and desensitize slowly. Moreover, the tonic current is highly sensitive to enhancement by amnestic drugs. Given the restricted expression of α5GABA A Rs to the hippocampus and the association between reduced α5GABA A R function and improved memory performance in behavioral studies, our results suggest that tonic inhibition mediated by α5GABA A Rs in hippocampal pyramidal neurons plays a key role in cognitive processes.