SIFT web server: predicting effects of amino acid substitutions on proteins

TLDR

The SIFT algorithm, introduced in 2001, predicts the functional impact of coding variants and has become a standard tool for characterizing missense mutations, with its web server hosted at FHCRC, JCVI, and currently BII. The authors present accuracy metrics evaluated on independent datasets. The updated SIFT tool now includes genome‑wide predictions and new features for indel analysis. The web server is available at http://sift-dna.org.

Abstract

The Sorting Intolerant from Tolerant (SIFT) algorithm predicts the effect of coding variants on protein function. It was first introduced in 2001, with a corresponding website that provides users with predictions on their variants. Since its release, SIFT has become one of the standard tools for characterizing missense variation. We have updated SIFT’s genome-wide prediction tool since our last publication in 2009, and added new features to the insertion/deletion (indel) tool. We also show accuracy metrics on independent data sets. The original developers have hosted the SIFT web server at FHCRC, JCVI and the web server is currently located at BII. The URL is http://sift-dna.org (24 May 2012, date last accessed).