Chlorophyll <i>a</i> Appearance in the Dark in Higher Plants: Analytical Notes

Abstract

The increase in the ratio [chlorophyll a]/[chlorophyll b] which occurs in expanding bean leaves in the dark, is a result of chlorophyll a formation with a concomitant loss in chlorophyll b. The analytical methods for assay of these pigments were examined closely and found to be adequate for this purpose when proper safeguards are taken.