Solid‐State NMR Spectroscopy Reveals that <i>E. coli</i> Inclusion Bodies of HET‐s(218<b>–</b>289) are Amyloids

Abstract

Protein deposition frequently occurs as inclusion bodies (IBs) during heterologous protein expression in E. coli. The structure of these E. coli IBs of the prion-forming domain from the fungal prion HET-s is the same as that previously determined for fibrils assembled in vitro, and show prion infectivity. These results demonstrate that the IBs of HET-s(218-289) are amyloids.