Diversity of Chloroplast DNA in Various Mandarins (Citrus spp.) and Other Citrus Demonstrated by CAPS Analysis

Abstract

The diversity of chloroplast DNA (cpDNA) of citrus was revealed by means of a cleaved amplified polymorphic sequence (CAPS) using 97 Citrus, including many local accessions from Japan, China, and Indonesia, four Fortunella, and two Poncirus. All accessions were classified into seven types based on the results of polymorphic bands in all primer/enzyme combinations. The seven types were identified as follows: Type 1 (38 accessions): C. macroptera, C. hystrix, C. aurantifolia, C. medica, C. limon, C. grandis, C. aurantium, C. myrtifolia, C. bergamia, C. rokugatsu, C. sinensis, C. sphaerocarpa, C. nobilis (Kunenbo), C. keraji, C. oto, C. tarogayo, C. suavissima, mandarins of Yunnan and Guangxi, China, miscellaneous of Yunnan, China, C. madurensis, and Fortunella; Type 2 (one accession): C. latipes; Type 3 (two accessions): C. ichangensis and C. junos; Type 4 (six accessions): C. tachibana and C. depressa; Type 5 (18 accessions): C. jambhiri, C. tankan, C. sunki, C. reshni, C. depressa, mandarins of Kagoshima, Japan and Yunnan and Guangxi, China; Type 6 (36 accessions): C. nobilis (King), C. unshiu, C. reticulata, C. genshokan, C. clementina, C. succosa, C. suhuensis, C. tardiferax, C. erythora, C. kinokuni, C. oleocarpa, C. leiocarpa, mandarins of Kagoshima, Japan, Guangxi, Zejiang, Yunnan and Guangdone, China, and mandarins of West Sumatra, Indonesia; Type 7 (Two accessions): Poncirus. The differentiation of cpDNA in citrus was discussed in accordance with the results.