PubMed · 2008 · 58 citations · 9 references
Life SciencesInfectious DiseasesPoultry ScienceMolecular VirologyPoultry HealthVirologyPoultry Infectious DiseasesPoultry VirologyVeterinary VaccinesViral Diseases Of PoultryViral GeneticsViral VaccinesAvian Influenza VaccinesAvian Influenza VirusAvian Influenza VirusesAvian Virus VaccinesGenome ReassortmentPoultry Vaccine DevelopmentPoultry VaccinesVaccine Strain Selection
To establish vaccine strains of H5 and H7 influenza viruses, A/duck/Hokkaido/Vac-1/04 (H5N1) [Vac-1/04 (H5N1)], A/duck/Hokkaido/Vac-3/07 (H5N1) [Vac-3/07 (H5N1)], and A/duck/Hokkaido/ Vac-2/04 (H7N7) [Vac-2/04 (H7N7)] were generated from non-pathogenic avian influenza viruses isolated from migratory ducks. Vac-1/04 (H5N1) and Vac-3/07 (H5N1) were generated by genetic reassortment between H5N2 or H5N3 virus as an HA gene provider and H7N1 or H6N1 viruses as an NA gene provider. Vac-2/04 (H7N7) was a genetic reassortant obtained using H7N7 and H9 N2 viruses to give high growth character of the H9N2 virus in chicken embryonated eggs. The results of sequence analyses and experimental infections revealed that these H5N1 and H7N7 reassortant viruses were non-pathogenic in chickens and embryos, and had good growth potential in embryonated eggs. These viruses should be useful to develop vaccines against H5 and H7 highly pathogenic avian influenza viruses.
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Universal primer set for the full-length amplification of all influenza A viruses
E. Hoffmann, Jürgen Stech, Yi Guan et al. · Archives of Virology · 2001 · 2.1K citations · Full text
Influenzavirus neuraminidase and neuraminidase-inhibition test procedures.
M Aymard-Henry, M. T. Coleman, W. R. Dowdle et al. · PubMed · 1973 · 421 citations