Removal of Feedback Inhibition of Δ1-Pyrroline-5-Carboxylate Synthetase Results in Increased Proline Accumulation and Protection of Plants from Osmotic Stress

TLDR

The rate‑limiting enzyme Δ1‑pyrroline‑5‑carboxylate synthetase (P5CS) is feedback‑inhibited by proline, a regulation that may be lost in plants under stress. Transgenic tobacco expressing a feedback‑inhibition‑deficient mutant P5CSF129A was compared to plants expressing wild‑type Vigna aconitifolia P5CS to assess proline accumulation. Plants with P5CSF129A accumulated twice as much proline, especially under 200 mM NaCl, which lowered malondialdehyde levels and enhanced growth in saline medium, confirming that P5CS feedback control regulates proline levels and protects against osmotic oxidative stress.

Abstract

Abstract The Δ1-pyrroline-5-carboxylate synthetase (P5CS; EC not assigned) is the rate-limiting enzyme in proline (Pro) biosynthesis in plants and is subject to feedback inhibition by Pro. It has been suggested that the feedback regulation of P5CS is lost in plants under stress conditions. We compared Pro levels in transgenic tobacco (Nicotiana tabacum) plants expressing a wild-type form of Vigna aconitifolia P5CS and a mutated form of the enzyme (P5CSF129A) whose feedback inhibition by Pro was removed by site-directed mutagenesis. Transgenic plants expressing P5CSF129A accumulated about 2-fold more Pro than the plants expressing V. aconitifolia wild-type P5CS. This difference was further increased in plants treated with 200 mm NaCl. These results demonstrated that the feedback regulation of P5CS plays a role in controlling the level of Pro in plants under both normal and stress conditions. The elevated Pro also reduced free radical levels in response to osmotic stress, as measured by malondialdehyde production, and significantly improved the ability of the transgenic seedlings to grow in medium containing up to 200 mm NaCl. These findings shed new light on the regulation of Pro biosynthesis in plants and the role of Pro in reducing oxidative stress induced by osmotic stress, in addition to its accepted role as an osmolyte.