The Journal of Biochemistry · 1972 · 36 citations · 1 references
Life SciencesBiochemistryMicrobiologyBiophysical ChemistryEnzyme MechanismEnzymologyBioluminescenceCatalytic MechanismsEnzyme BiophysicsEnzyme KineticsPhotocatalysisPhotochemistryBioluminescent Reaction MechanismsBioluminescent ReportersFluorescence Quantum YieldLuciferase EnzymologyPhotochemical Quantum YieldPhotosynthetic BacteriaPhotosynthetic EnzymesSubstrate Binding Specificity
The substrate specificity and stoichiometry of the reaction of a bacterial luciferase from Photobacterium phosphoreum were studied. This enzyme shows only negligible activity with FADH2 and reduced riboflavin, compared with that with FMNH2. Aliphatic aldehydes of longer chain length have higher affinity for the enzyme and caused a greater increase in the quantum yield of the bioluminescent reaction. The stoichiometry of the luciferase reaction was found to be luciferase: FMNH2: O2= 1: 1: 1.
1
The Luminescence of Biological Systems
Vladimir Walters, Frank H. Johnson · Copeia · 1958 · 95 citations