Infection and Immunity · 2001 · 74 citations · 14 references
Pathogenic MicrobiologyMicrobial PathogensBacteriologyImmunologyMolecular BiologyBacterial PathogensInfection ControlSerotype 2Antimicrobial ResistancePorcine ModelVirulence FactorMolecular MicrobiologyClinical MicrobiologyDefined MutantSly GenePathogenesisMicrobiologyMedicineMicrobial Genetics
A defined allelic-replacement mutant of the sly gene, encoding a thiol-activated cytolysin, from a European isolate of Streptococcus suis serotype 2 was generated and characterized. Unlike the parental strain, it is nonhemolytic, noncytotoxic for cultured macrophage-like cells, avirulent in a mouse infection model, yet only slightly attenuated in a porcine model of systemic infection.
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