Searching for Gene Defects by Denaturing Gradient Gel Electrophoresis

Abstract

In attempting to ascertain the presence of particular alleles of critical genes in a human fetus, it would be most desirable to know precisely the base sequence of its genome. It is not clear how soon direct sequencing techniques will become fast and easy enough to permit scrutiny of 50 kb within the limits of Medicare reimbursement set by the diagnostic related groupings (DRGs), but for the present, other paths to detection of departures from normal sequence seem to be necessary.