Autonomous Fueled Mechanical Replication of Nucleic Acid Templates for the Amplified Optical Detection of DNA

Abstract

Fuel for a DNA machine: A FokI enzyme/DNA scission machine is triggered by the hybridization of its DNA hairpin structure with a target nucleic acid. The machine is fueled by a secondary fluorophore (F)- and quencher (Q)-functionalized nucleic acid 2. Scission of 3 leads to replication of the machinery cutter 1 and yields a fluorescent product 6, which provides an optical output for the machine (see scheme). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2006/z503810_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.