A Bacterial Artificial Chromosome Library of <i>Lotus japonicus</i> Constructed in an <i>Agrobacterium tumefaciens</i>-Transformable Vector

Abstract

We constructed a BAC library of the model legume Lotus japonicus with a 6-to 7-fold genome coverage. We used vector PCLD04541, which allows direct plant transformation by BACs. The average insert size is 94 kb. Clones were stable in Escherichia coli and Agrobacterium tumefaciens.