Multicenter Evaluation of a New Shortened Peptide Nucleic Acid Fluorescence <i>I</i> <i>n</i> <i>S</i> <i>itu</i> Hybridization Procedure for Species Identification of Select Gram-Negative Bacilli from Blood Cultures

Abstract

A shortened protocol for two peptide nucleic acid fluorescence in situ hybridization (PNA FISH) assays for the detection of Gram-negative bacilli from positive blood cultures was evaluated in a multicenter trial. There was 100% concordance between the two protocols for each assay (368 of 368 and 370 of 370 results) and 99.7% (367 of 368 and 369 of 370 results) agreement with routine laboratory techniques.