The evaluation of micronucleus frequency by acridine orange fluorescent staining in peripheral blood of rats treated with lead acetate

Abstract

The data concerning the mutagenic, clastogenic and carcinogenic properties of inorganic lead compounds have been conflicting. Here, we evaluated the frequency of micronuclei in the peripheral blood of female rats treated with three different lead acetate doses. Outbred female Wistar rats were treated by gavage once per week for 10 weeks with cumulative doses of 140, 250 and 500 mg/kg body weight (body wt) of lead acetate. Mitomycin C (MMC) 2 mg/kg body wt was used as a positive control. The aim of the present study was to investigate the possible cytotoxic and genotoxic effects of lead acetate on peripheral blood reticulocytes using the micronucleus test following chronic exposure. The results show the effects of lead acetate in peripheral blood reticulocytes. These effects are both cytotoxic and genotoxic because of a decrease in the number of polychromatic erythrocytes in the peripheral blood and an increase in frequency of micronucleated reticulocytes, respectively.