Spectral polarization imaging of human rectum-membrane-prostate tissues

Abstract

Human rectum-membrane-prostate tissue samples were studied using a near-infrared spectral polarization imaging technique to detect small objects and structural changes inside prostate tissues through the rectum. Four modeling samples were made with a small piece of absorber or prostate tissue dyed with a contrast agent (indocyanine green) embedded inside a large piece of prostate tissue in rectum-membrane-prostate structures. The depth of the foreign objects underneath the surface of the rectum-membrane-prostate structures was varied from a millimeter to a centimeter to obtain the critical imaging distance. Different spectral polarization imaging methods with and without contrast agents were performed and compared. The results show that small objects hidden inside the host prostate tissues in the rectum-membrane-prostate structures at depths of 2.5, 4.0, and 7.5 mm can be imaged and identified using the scattering light imaging, tissue emission wing imaging, and contrast agent fluorescence imaging methods, respectively. Our results indicate the potential of imaging and detecting structural changes and cancers inside prostate tissue though rectum-membrane-prostate tissues using this noninvasive spectral polarization imaging technique.