Acta Oto-Laryngologica · 1993 · 86 citations · 23 references
Life SciencesInfectious DiseasesMicrobiologyMolecular VirologyNeuropathologyVirologyNeuropathology Of InfectionVestibular NeuritisVestibular NeurobiologyVestibular PhysiologyViral InfectionsViral PathologyDna In Situ HybridizationHerpes Simplex Virus LatencyHerpes Simplex Virus ReactivationHerpesvirus ReactivationImmunohistochemical DetectionImmunohistochemical StainingLatency-associated TranscriptsViral Etiology
Viral infection has been considered to be a possible pathogenesis of vestibular neuronitis, and reactivation of the herpes simplex virus (HSV) is one of the most likely causes. However, it remains unknown whether the human vestibular ganglia contain latent HSV. We examined 26 vestibular ganglia from autopsied adults in search of HSV type 1 (HSV-1). To detect HSV-1, we used polymerase chain reaction (PCR), in situ hybridization and immunohistochemical staining. HSV DNA was detected in 6 of 10 vestibular ganglia using the PCR method. However, the latency-associated transcript (LAT) of HSV-1 was negative in all of the 16 vestibular ganglia examined. No HSV antigen was detected in any of the ganglia. These results indicate that HSV-1 is latently infected in the human vestibular ganglia, and that LAT is transcribed weakly or not at all.
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Tom Maniatis · Medical Entomology and Zoology · 1989 · 1.2K citations
RNA Complementary to a Herpesvirus α Gene mRNA Is Prominent in Latently Infected Neurons
J G Stevens, E K Wagner, G B Devi-Rao et al. · Science · 1987 · 904 citations