Improved Detection of <i>Bartonella</i> DNA in Mammalian Hosts and Arthropod Vectors by Real-Time PCR Using the NADH Dehydrogenase Gamma Subunit ( <i>nuoG</i> )

Abstract

ABSTRACT We used a whole-genome scanning technique to identify the NADH dehydrogenase gamma subunit ( nuoG ) primer set that is sensitive and specific enough to detect a diverse number of Bartonella species in a wide range of environmental samples yet maintains minimal cross-reactivity to mammalian host and arthropod vector organisms.