Two‐Photon Fluorescent Probes for Intracellular Free Zinc Ions in Living Tissue

Abstract

The living image: The efficient two-photon probes AZn1 and AZn2 (see picture for rat brain tissue) show 24- to 52-fold two-photon excited fluorescence enhancement in response to Zn2+. They can selectively detect intracellular free Zn2+ ions in live cells and in living tissues at a depth of 80–150 μm without interference from other metal ions and the membrane-bound probes. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z800929_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.