Carvacrol and thymol components inhibiting Pseudomonas aeruginosa adherence and biofilm formation

Abstract

Pseudomonas aeruginosa has a high propensity to develop biofilms that are resistant to exogenous deleterious agents. The aim of this study was to investigate whether carvacrol and thymol can interfere with adherence phenomena as well as acting on biofilm formation. Tests of P. aeruginosa strains showed that carvacrol and thymol interferes with the starting phases of adherence as well as with P. aeruginosa biofilms. Carvacrol and thymol (2MIC) inhibition was 97±8.5 and 89±6.3% for P. aeruginosa (ATCC 27853)and 72±4.6 and 69±6.8% for P. aeruginosa (CIP A22) adherence respectively. Carvacrol (2MIC) inhibition exceeds 90% for P. aeruginosa (ATCC 27853) and P. aeruginosa (IL5)biofilm. Thymol (2MIC) inhibition is 86±2.1, 54±5.9 and 70±4.3% for P. aeruginosa (ATCC 27853) P. aeruginosa (CIP A22), P. aeruginosa (IL5), respectively.   Key words: Biofilm, adherence, carvacrol, thymol, Pseudomonas aeruginosa.