Zenodo (CERN European Organization for Nuclear Research) · 2010 · 11 citations · 10 references
Life SciencesPlant BiologyPlant SciencesEmbryogenesisEmbryologyPlant Developmental BiologyEmbryo CultureEmbryo DevelopmentEmbryonic GrowthSomatic EmbryogenesisAuxin TreatmentsCallus MaturationEmbryo Development In FruitEmbryogenic Callus InductionEmbryogenic Callus TransformationMature Embryo CultureMicropropagation Of RootstocksSomatic Embryo Induction
Nucellar embryogenesis was induced in Mangifera indica L. cv. Kurakkan, a polyembryonic salt tolerant, dwarfing rootstock. Nucellus tissue excised from 3.5 cm long fruits developed pro-embryonic callus in 19 days of inoculation on modified MS medium supplemented with 4.52μM 2,4- D, 0.05% malt extract and 13.78μM spermidine. Somatic embryogenesis exhibited high frequency (158.33 embryos). However, all the differentiated embryos proliferated on medium having low level of sucrose (4% w/v) and auxin (2.26μM 2,4-D). Most of the proembryonic calli converted into heart shaped and cotyledonary embryos by reducing temperature to 15 oC. Somatic embryos were matured on modified MS medium fortified with 0.38μM ABA, 0.57μM IAA and 30.30μM PEG. Matured somatic embryos germinated (around 30%) on MS medium supplemented with 2.68μM NAA, 11.60μM kinetin and 2736.9μM glutamine. This record was migrated from the OpenDepot repository service in June, 2017 before shutting down.
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