Indirect Immunofluorescence Assay for <i>Intra Vitam</i> Diagnosis of Avian Bornavirus Infection in Psittacine Birds

Abstract

Different avian bornavirus (ABV) genotypes have recently been detected in psittacine birds with proventricular dilatation disease (PDD), an inflammatory fatal central and peripheral nervous system disorder. An indirect immunofluorescence assay (IIFA) for intra vitam demonstration of ABV-specific serum antibodies was established since reverse transcription-PCR (RT-PCR) assays may not detect all ABV variants.