3D EXAFS refinement of the Cu site of azurin sheds light on the nature of structural change at the metal centre in an oxidation–reduction process: an integrated approach combining EXAFS and crystallography

Abstract

Three-dimensional information is obtained for the Cu site in azurin at very high resolution by combining high-resolution crystallographic structures and EXAFS data for the oxidized and reduced form of the protein. This combined approach has allowed us to define the subtle structural changes (<0.1 A) which take place at the Cu site during a single-electron redox process.