Differential Use of the Two High-Oxygen-Affinity Terminal Oxidases of <i>Brucella suis</i> for In Vitro and Intramacrophagic Multiplication

Abstract

Expression of the high-oxygen-affinity cytochrome cbb3 and cytochrome bd ubiquinol oxidases of Brucella suis was studied in vitro and in the intramacrophagic niche, which was previously proposed to be oxygen limited. The cytochrome cbb3 oxidase was exclusively expressed in vitro, whereas the cytochrome bd oxidase was preferentially used inside macrophages and contributed to intracellular bacterial replication.