Assessment of the Neutrophil Dominating Protein Calprotectin in Feces: A Methodologic Study

TLDR

We have content for all. Need to summarize each. Background: "This protein is a prominent antimicrobial component of neutrophils, monocytes, macrophages, and squamous epithelia." So: "Calprotectin is a key antimicrobial protein found in neutrophils, monocytes, macrophages, and squamous epithelia." That covers. Purpose: "AbstractThis study describes methods for extraction and quantification of calprotectin (LI protein) in feces by enzyme immunoassay." So: "The study aims to develop and validate methods for extracting and measuring fecal calprotectin using enzyme immunoassay." Good. Mechanism: same line: "AbstractThis study describes methods for extraction and quantification of calprotectin (LI protein) in feces by enzyme immunoassay." So: "Methods involved extracting calprotectin from fecal samples and quantifying it via enzyme immunoassay." Good.

Abstract

AbstractThis study describes methods for extraction and quantification of calprotectin (LI protein) in feces by enzyme immunoassay. This protein is a prominent antimicrobial component of neutrophils, monocytes, macrophages, and squamous epithelia. Calprotectin was stable in feces during storage for 7 days at room temperature. Small fecal samples taken from a 24-h feces collection gave a reliable estimate of calprotectin. Within-assay precision was 1.9%, and between-assay precision 14.8%. In healthy subjects (n = 33) median fecal calprotectin was 2025 μ/l and in hospital controls (n = 40) 10,500 ug/1. Median values in patients with Crohn's disease (n = 21) was 43,000 u.g/1 and in ulcerative colitis (n = 17) 40,000 μg/1. Fecal calprotectin was significantly correlated to fecal α1-antitrypsin in the patients with Crohn's disease. Ten of 11 patients with gastrointestinal carcinomas had calprotectin level above the suggested reference limit of 6740 μg/1.Key Words: Fecal calprotectingastrointestinal carcinomainflammatory bowel disease activityleukocyte turnover