Ultracentrifugal Fractionation of Bovine Nasal Chondromucoprotein

Abstract

SUMMARY Bovine nasal chondromucoprotein can be separated cen- trifugally into two distinct fractions, each of which is composed of protein and polysaccharide. The light fraction obtainable in yields of 75’+& consists of 15oj, protein and 85% polysaccharide. It sediments as a single component and has a sedimentation constant at infinite dilution of 10.5 S. The heavy fraction, easily sedimentable, and obtainable in yields of 13oj,, consists of about 50% protein and 50% polysaccharide, and sediments completely at 10,000 X g. It is not clear whether the heavy fraction consists of a single component. Degradation of both the heavy and the light fractions with alkali appears to yield the same polysaccharide, chondroitin sulfate A. REFERENCES 1. SHATTON, J., AND SCHUBERT, M., J. Biol. Chem., 211, 565 (1954). 2. MALAWISTA, I., AND SCHUBERT, M., J. Biol. Chem., 230, 535 (1958). 3. WARNER, R. C., AND SCHUBERT,