PHOTOSYNTHETIC FUNCTION IN <i>DUNALIELLA TERTIOLECTA</i> (CHLOROPHYTA) DURING A NITROGEN STARVATION AND RECOVERY CYCLE

Abstract

Phytoplankton can be exposed to periods of N starvation with episodic N resupply. N starvation in Dunaliella tertiolecta (Butcher) measured over 4 days was characterized by slow reduction in cell chl and protein content and chl/carotenoid ratio and a decline in photosynthetic capacity and maximum quantum yield of photosynthesis (F v /F m ). In the early stages of N starvation, cell division was maintained despite reduction in cellular chl. Chl content was more sensitive than carotenoids to N deprivation, and cellular chl a was maintained preferentially over chl b under N starvation. NO 3 − resupply stimulated rapid and complete recovery of F v /F m (from 0.4 to 0.7) within 24 h and commencement of cell division after 10 h, although N‐replete levels of cell chl and protein were not reestablished within 24 h. Recovery of F v /F m was correlated with increases in cell chl and protein and was more related to increases in F m than to changes in F 0 . Recovery of F v /F m was biphasic with a second phase of recovery commencing 4–6 h after resupply of NO 3 − . Uptake of NO 3 − from the external medium and the recovery of F v /F m , cell chl, and protein were inhibited when either cytosolic or chloroplastic protein synthesis was inhibited by cycloheximide or lincomycin, respectively; a time lag observed before maximum NO 3 − uptake was consistent with synthesis of NO 3 − transporters and assimilation enzymes. When both chloroplastic and cytosolic translation was inhibited, F v /F m declined dramatically. Dunaliella tertiolecta demonstrated a capacity to rapidly reestablish photosynthetic function and initiate cell division after N resupply, an important strategy in competing for limiting inorganic N resources.