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One-Step Immunochromatographic Dipstick Tests for Rapid Detection of <i>Vibrio cholerae</i> O1 and O139 in Stool Samples

Farida Nato, A Boutonnier, Minoarisoa Rajerison, Pierre Grosjean, Sylvie Dartevelle, Alain Guénolé, N. A. Bhuiyan, David A. Sack, G. Balakrish Nair, Joëlle Fournier,

Clinical and Vaccine Immunology · 2003 · 102 citations · 14 references

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Abstract

We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae O1 and O139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.

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