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Production de plantes haploïdes de Gerbera <i>jamesonii</i> par culture <i>in vitro</i> d'ovules
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1986
Year
BiologyEngineeringBotanyNatural SciencesMicropropagationPlant ReproductionBiotechnologyGenetic EngineeringAgricultural EconomicsPlant Cell CulturePlant ProductionBenzyl AdenineHorticultural PlantPlant PhysiologyGerbera JamesoniiIndole Acetic Acid
Unfertilized ovules of Gerbera jamesonii were cultivated in vitro and haploid plants were successfully regenerated. Five concentrations of sugar, and four hormonal combinations containing indole acetic acid and benzyl adenine were tested. The best results were obtained with 0.1 mg indole acetic acid/L and 0.2 mg benzyl adenine/L; 1% sugar in the medium was sufficient for callus production and regeneration of plantlets from the clone of the experimental cultivar, 'Super Gerbera'. One hundred and fifty ovules per capitulum can be cultivated and under optimal conditions a frequency of 5% haploid plantlets can be obtained.